Name，Zou Yongxin
Associate Prof
Telephone:531-8838-2043
E-mail: zouyongxin@sdu.edu.cn
Education and qualifications：
1999-2003 School of Life Science, Shandong University (MD)
2003-2009School of Medicine, Shandong University(PhD)
2009-2013 Research Fellow, Department of School of Medicine, Shandong University
2012-2014 Postdoctoral fellow, Department ofLife Science,Hong Kong University of Science and Technology
2013- Associate Professor, ShandongUniversity
Research programme：Cell cycle and DNA replication regulation
Research Interest：
Complete and precise replication of the DNA is one of the critical events in the cell cycle. In eukaryotic cells, the initiation of DNA replication is tightly regulated. During this process, pre-replication complexes (pre-RCs) assemble and bind to replication origins and proper DNA replication depends largely on the regulation on the formation of pre-RC. Although several of proteins have been identified, the discovery of novel regulatory proteins will provide important insights into the mechanisms of the DNA replication and cell cycle.
In our previous research we found the level of replication initiation factor CDC6 was positively correlated to that of CUL4B. The CUL4B contributes to the maintenance of CDK2 at the post-transcriptional level through modulation of the microRNAs expression (miRNAs), which is in turn responsible for phosphorylation and stabilization of CDC6. These findings suggest that CUL4B has a novel function in regulating DNA replication by maintaining the structural integrity of pre-replication complexes. This is the first report that cullins may function in the regulation of miRNAs. Our findings provide a new insight into how CUL4B regulates cell cycle progression and DNA replication.
Grants
1.Mechanistic study of miRNA-mediated regulation of cell cycle by CUL4B. National Science Foundation Research grants
2.Study of role of CUL4B, an mental retardation gene, in cell cycle regulation. National Science Foundation Research grants
3.Roles of hCdc14 in DNA replication initiation.China Postdoctoral Science Foundation funded project2013M530317
4.Novel anticancer agents against human DNA replication-initiation proteins with low toxicityHong Kong Scholars Program 201104631
Representative Publications
1.Zou Y,Mi J, Wang W , Lu J , Zhao W, Liu Z, Hu H, Yang Y , Gao X, Jiang B, Shao C*, Gong Y*. CUL4B promotes replication licensing by up-regulating the CDK2–CDC6 cascade.J Cell Biol.2013 Mar 18;200(6):743-56.
2.Zou Y,Mi J, Cui J, Lu D, Zhang X, Guo C, Gao G, Liu Q, Chen B, Shao C*, Gong Y*. Characterization of nuclear localization signal in the N terminus of CUL4B and its essential role in cyclin E degradation andcell cycle progression.J Biol Chem.2009, 284(48):33320-33332.
3.Zou Y,Liu Q, Chen B, Zhang X, Guo C, Zhou H, Li J, Gao G, Guo Y, Yan C, Wei J, Shao C, Gong Y*. Mutation in CUL4B, which encodes a member of cullin-RING ubiquitin ligase complex, causes X-linked mental retardation.Am J Hum Genet.2007; 80(3):561-566
4. Mi, J#.,Zou, Y#., Lin, X., Lu, J., Liu, X., Zhao, H., Ye, X., Hu, H., Jiang, B., Han, B., Shao, C., Gong, Y. Dysregulation of the miR-194-CUL4B negative feedback loop drives tumorigenesis in non-small-cell lung carcinoma.Molecular oncology.2017. 11, 305-319
